Generator
pRha-B0034
Part:BBa_K902067:Design
Designed by: Himika Dastidar Group: iGEM12_Calgary (2012-10-02)
Rhamnose promoter with S7 micrococal nuclease
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This construct was designed as part of a kill switch for 2012 iGEM Calgary project. Please view our [http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch Wiki] for more details about our kill system. This circuit needs to be grown up on special LB plates containing >0.2% glucose to repress this system successfully.
Source
The pRha promoter and S7 nuclease were commercially synthesized. The RBS was taken from the Parts Registry.
References
Dingwall C, Lomonossoff GP, Laskey RA. High sequence specificity of micrococcal nuclease. Nucleic Acids Res 1981 Jun 25;9(12):2659-2673.